Laser microdissection lmd allows the isolation of individual cells or chromosomes and is a well established technique for sample preparation prior to downstream analysis of the nucleic acid content via pcr or sequencing techniques here we describe the successful combination of the leica lmd system and qiagen kits for purification of nucleic acids even from small amounts. Laser capture microdissection under a microscope using a software interface a tissue section typically 5 50 micrometres thick is viewed and individual cells or clusters of cells are identified either manually or in semi automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. Medicina article validation of laser capture microdissection protocol in endometriosis studies katiane de almeida da costa 1 helena malvezzi 1 bruno gallani viana 1 renée zon filippi 1 rosa maria neme 1 2 thiago pinheiro arrais aloia 1 sérgio podgaec 1 and carla de azevedo piccinato 1 1 hospital israelita albert einstein são paulo br 05652 900 brazil 2 centro de endometriose são.
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